and has been reported to possess antioxidant properties both in in vitro and animal studies. 4. For this purpose DPPH (2,2-diphenyl-1-picrylhydrazyl), ORAC (oxygen radical absorbance capacity) and TPC (total phenolic content) assays together with MTT cell viability assay (performed on . Here, 70% ethanol extracts of 22 HPs collected from along the coast of South Korea were investigated for their potentials of antioxidant, anti-aging, and whitening properties for use as materials in novel cosmeceuticals. 2.4 | In vitro antioxidant assays 2.4.1 | DPPH scavenging activity The method of Shen et al. MIC and MBC concentrations of four oil- or water-soluble rosemary (Rosmarinus officinalis) extracts against gram-positive (Bacillus and Staphylococcus) and gram-negative (Campylobacter and Salmonella) bacteria were determined by using disk diffusion, agar dilution, and broth . World Scientific News 171 (2022) 1-20 -3- Gmelina arborea extracts to cure diarrhea.This study aims to examine the phytochemicals and in vitro antioxidant activity of Gmelina arborea leaf extracts.Moreover, to determine the phytochemicals that are responsible for therapeutic effects, HPTLC was used to profile For this purpose DPPH (2,2-diphenyl-1-picrylhydrazyl), ORAC (oxygen radical absorbance capacity) and TPC (total phenolic content) assays together with MTT cell viability assay (performed on . In this study, aqueous extracts of some medicinal and aromatic plants (garlic, Turkish Oregano, rosemary, basil, and peppermint) were used in probiotic yogurt production to increase functionality of probiotic yogurt. Journal of Plant Science and Research 2013. The evaluation of the antimicrobial potential of both methanolic and ethanolic extracts of three fruit . Evaluation of In Vitro Antioxidant and Antidiabetic Activities of . The reaction mixture consists of 1 ml of plant extract (1mg/ml), 1 ml of PMS (60m) preprared in Reducing power assay The reducing power of E. agallocha was determined as per the reported method . In the Far North Region of Cameroon, pastoralists use the leaves of Boscia senegalensis and the stem of Tapinanthus dodoneifolius to treat common animal diseases. The use of plant peels has dual benefits; first is their antimicrobial activity against food-borne pathogens, while the second is minimizing agro-industrial wastes. The protective effects of plant extracts were compared with a well known antioxidant, Butilated Hydroxytoluen (BHT) and -tocopherol. Although many of these species are nearly facing extinction . SCF extracts were subjected to column chromatography was resulted in six fractions.
The ABTS method is known to be a rapid method for the determination of the antioxidant activity and could be a useful tool to screen samples and cultivars in order to obtain high content of natural antioxidants in foods .The ABTS + scavenging activity is presented in the Table 4.This results show that at all the concentration, the AEE showed a higher inhibitory potential compared to other . In this study, the ethanolic extract of the plant C. maritima was used for the determination of TPC, TFC, antioxidant activity, and ex vivo anti-cataract activity using goat eye lenses to know its potential for the effective treatment of cataract. in methanol extract and lesser activity in aqueous extract at 5 mg concentration.
The germination of both Bacillus spp. Search results . 2. comparative study on phytochemical screening and antioxidant activity on ethanolic extract of actinidia deliciosa and vitis vinfera qr code. To achieve this, four extracts (water, methanol, chloroform and hexane) of both plants obtained by successive . Regarding this ethnopharmacological study allowed to compare the antimicrobial activities of various plant species, to establish the antimicrobial spectra of standardized plant extracts, and give support to the traditional use of these plants. Ismail Hossain, Farzana Anwar Sharmin, Sadika Akhter, Mohiuddin Ahmed Bhuiyan, *Mohammad Shahriar Department of Pharmacy, University of Asia Pacific, Dhanmondi, Dhaka, Bangladesh INTRODUCTION For decades, the utilization of herbal plants has The antioxidant activity of plant extracts was determined by different in vitro methods such as the DPPH free radical scavenging assay and reducing power methods. in vitro anti-oxidant activity of ethanolic extract of grewia flavescens whole plant extract. Out of twenty plant extracts tested, eight showed significant potency against RVFV indicated by a decrease in tissue culture infectious dose (TCID50) < 105. Inhibition of Acetylcholinesterase (AChE) is still considered as the main therapeutic strategy against Alzheimer's disease (AD). Methods: Polyphenols extraction was . In vitro antioxidant and anti-proliferative activity of Ethiopian medicinal plant extracts. Jamuna S, Paulsamy S, Karthika K. Screening of in vitro 22. ).pdf" See all downloads; Add to list . Generally, plant- or fruit/vegetable-derived foodstuffs are rich in phenols and exhibit an excellent antioxidant capacity, partic- ularly phenols that can give the hydrogen atoms in their hydroxyl groups (27). Duraipandiyan V, Ignacimuthu S. Antifungal activity of antioxidant activity of methanolic leaf and root extracts of traditional medicinal plants from Tamil Nadu, India. The antioxidant activities (AA), reducing powers (RP) and total phenolic contents (TPC) of methanol and water extracts of three lichen species, Usnea longissima Ach., Usnea florida (L.) Weber ex Wigg. Free radical scavenging activity The percentage (%) free radical 1,1-diphenyl-2-picrylhy- drazyl (DPPH) scavenging activity of extracts was assessed as previously described by Hatano et al  and was calculated as follows: % radical scavenging effect Abs1 Abs2 =Abs1 100 where Abs1 is the absorbance of the control, and Abs2 is the . The obtained results . However, the activity of the extract (0.84510.001) was lower than the standard (1.07940.010). Of the extracts tested, the methanol e Material and Methods: The plant extract (50mg) obtained by soxhlet extraction method was dissolved separately in 50ml of methanol and the resultant solution serially diluted to concentrations 0.5, 0.25, 0.125 and Both aqueous extracts inhibited -glucosidase activity in vitro; this inhibition of the enzyme could slow down the breakdown of disaccharide to simple glucose, hence reducing the amount of glucose absorbed in the blood (Kwon et al., 2006). Recently, numerous studies reported the antioxidant properties of medicinal plant products using DPPH assay [42-44]. The antioxidant activity was evaluated by determining the activity of hydrogen peroxide (H 2O2) radicals scavenging and 1,1-diphenyl-2-picryl hydrazyl (DPPH) assay. Antioxidative activities of plant extracts were found Many plant derived phytochemicals have shown AChE inhibitory activity in addition to the currently approved drugs for AD. by editor iajps. . aqueous) of the plant Glycosmis pentaphylla correa. In Vitro analysis of Different extracts of Carica papaya (leaves) and their Phytochemical constituents, Antibacterial activity and Antioxidant activity June 2022 DOI: 10.15515/abr.0976-4585.12.4 . Estimation of total phenolic content Total pheno lic cont ent was est imated using the Folin-Ciocalteu method  . This study was c onducted to investigat e the antioxidant effect of the differen t crud e extrac ts (Pe troleum e ther, e thanolic a nd. So far, in India, experimental studies have been conducted by a group of scientists, Anitha et al . All the assays was carried out in triplicate and average value were recorded. This study aimed to evaluate the in vitro antibacterial, non-cytotoxic and antioxidant potentials of these plants. download download pdf. 21,5 The high antioxidant activity of Ephedra alte extract can be explained by the presence of the hydroxyl groups in the phenolic compounds. The The antiproliferative and antioxidant potential of Curcuma aeruginosa extracts were investigated. Due to the climate diversity in Tunisia, the traditional pharmacopoeia consists of a wide arsenal of medicinal plant species since long used in folk medicine, in foods as spices, and in aromatherapy. Antioxidant activity was based on the absorbance on the final day of FTC method. Evaluation of antioxidant activity 4.1 Reducing power assay The reducing power of E. agallocha was determined as per the reported method (5). Theobroma cacao L. (Sterculiaceae) leaves have been used in folklore medicine as treatment for wound healing, worm expellers and malaria cure. Phytochemical investigation was done using . editor iajps. Fifty micro liters of the plant extract in methanol, yielding 100g/ml respectively in ABSTRACT The free radical scavenging activities of n-hexane extract of the whole plant of Emilia sonchifolia was evaluated by employing various in vitro assay systems like DPPH radical scavenging activity, superoxide radical scavenging activity and hydrogen peroxide scavenging activity with IC50 values 180, 160 and 160 g/ml respectively. Free radicals are the primary cause of oxidative damage of biological molecules in the human body, and are related to coronary heart disease, aging, cancer, and dementia .We used the 2,2-diphenyl-2-picrylhydrazyl (DPPH) assay to determine the antioxidant activity of cocoa plant methanolic extracts, i.e., their ability to scavenge free radicals; concentrations of . Result from this assay clearly showed that all extract exhibited high antiradical activity towards DPPH radical. Fresh root . The antioxidant activity is expressed as the number of equivalents of ascorbic acid and was calculated by the following equation: A = (c V) / m 20 Scholars Research Library Mohammad Shahriar et al Annals of Biological Research, 2015, 6 (4):19-26 _____ Where, A = total content of Antioxidant compounds, mg/gm plant extract, in Ascorbic acid . Medicinal plants, as source of remedies, are widely used as alternative therapeutic tools for the prevention or treatment of many diseases. g. pramodini.
Cordiamyxa fruit extract exhibits low cytotoxicity, antibacterial activity, and antioxidant properties in vitro and can be developed for pharmaceutical and medical uses in the future. Yogurt . Scavenging activity was tested by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and the inhibitory effect on lipid peroxidation was examined by the ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods. The reducing power increased significantly (P<0.01) with increasing concentration of the extract. It is well known that the antioxidant activity of plant extracts containing polyphenol components is due to the capacity to be donors of hydrogen atoms or electrons and to capture the free radicals. 2. Total polyphenols and total antioxidant activity of the Dahlia x hybrida flower extracts. is traditionally known to be used as an anti-infective, expectorant, healer of wounds and against inflammatory and gastrointestinal diseases. Phenolic plant extracts are sources of natural bioactive compounds, which can inhibit the rate of food spoilage. extract showed increased reducing power with increase in concentration. The final extract thus obtained was used for in vitro antioxidants activity. Materials and Methods: Herbal extraction was carried out by Soxhlet method using chloroform, ethylacetate, methanol, ethanol, aqueous and hydroalcohol. The aim of this study was to screen various solvent extracts of whole plant of Torilis leptophylla to display potent antioxidant activity in vitro and in vivo, total phenolic and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations.. Material and methods. In this study, the antioxidant and anthelmintic activities of aqueous and ethanolic extracts of the leaves of Theobroma cacao were investigated. one of the stable free radicals, commercially available, nitrogen centered, and largely used for evaluat-ing scavenging activity of antioxidant standards and plant extracts with a characteristic absorbance at nm ,
Total antioxi-dant activity of ethanol extracts of plants were also tested by using ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods. Abs sample is the absorbance of DPPH radical + sample (i.e.extract or standard) Scavenging activity of superoxide anion: The scavenging activity of the superoxide anion was determined by the method of Yen and Chen 17. Herein, the CMF ethanol extract was also evaluated for antioxidant activity. From these assay several molecules from medicinal plants were . of antioxidants in treating diabetes and its complications through prevention of oxidative stress has been explained in different studies.20,24-26 Interestingly, the current study revealed that Bersama abyssinica leaves have strong in vitro antioxidant activities indicating that the plant could possess antidiabetic activity. extract had reducing power but not at same level and the activity is ranging from 42.24 - 62.65 % Statistical correlations have been studied between respectively at a concentration of 1mg ml-1 in total phenolic content and antioxidant activity reaction mixture. Halophyte plant (HPs), a salt-resistant flora, has been reported to provide several health benefits, but the knowledge of its cosmeceutical potential is still ambiguous. A detailed study was performed on the antioxidant activity . The recent studies have investigated that the antioxidant effect of plant products is mainly attributed to phenolic compounds such as flavonoids, phenolic acids, tannins etc.
Table 2. Background. Rutin was used as standard; 1mg/ml of plant extracts and standard were diluted to a range of concentrations (10-700g/ml in metha - nol). Antioxidant Activity. 500 l of Download "(. The results of the free radical scavenging activity of Argel extract and the authentic antioxidant L-ascorbic acid was summarized in Table (1). However, both extracts exhibited a dose-dependent enzyme inhibitory activity in the range of Recent investigation has shown that many flavonoids and related polyphenol contribute significantly to the antioxidant activity of many fruits such as red grape, vegetables and medicinal plants . It is known that the phenolic character of curcumin is responsible for its anti-oxidant properties . All seven plants (100%) inhibited the growth of at least one tested foodborne and spoilage bacteria and fungi. The fresh blood was collected and mixed with an equal volume of sterilized . The HRBC membrane stabilization method is mainly used to estimate the anti-inflammatory activity of plant extracts 110. 1,2 Accumulation of free radicals can cause pathological conditions such as . 552 nm. The protective effects of plant extracts were compared with a well known antioxidant, Butilated Hydroxytoluen (BHT) and -tocopherol. The in vitro antidiabetic, antioxidant activity, total phenolic compound content and phenolic compounds of yogurts were evaluated during the 28 day of storage period. Proteinase activity was also significantly inhibited by the leaf (84.19 g/ml) and stem (81.84 g/ml). Request PDF | In Vitro Antioxidant, Cytotoxic, Thrombolytic Activities and Phytochemical Evaluation of Methanol Extract of the Ampelocissus Barbata (Wall.) The antioxidant activity of methanolic extracts from Garcinia atroviridis obtained via maceration showed good results with a minimum EC 50 value of 9.32 and 5.32 g/mL for DPPH and ABTS assay . In the present study, methanolic extracts of 20 plants used in Indian Ayurvedic system of medicine for improving cognitive function were . This scavenging activity has been widely used as a quick and reliable parameter to evaluate the general in vitro antioxidant activity of plant extracts [40, 41]. Free radical scavenging activity The percentage (%) free radical 1,1-diphenyl-2-picrylhy- drazyl (DPPH) scavenging activity of extracts was assessed as previously described by Hatano et al  and was calculated as follows: % radical scavenging effect Abs1 Abs2 =Abs1 100 where Abs1 is the absorbance of the control, and Abs2 is the . Generally, plant- or fruit/vegetable-derived foodstuffs are rich in phenols and exhibit an excellent antioxidant capacity, partic- ularly phenols that can give the hydrogen atoms in their hydroxyl groups (27). . The results suggested that the strong antioxidant activity of extracts might be due to the presence of phenolics compounds present in the extract . 0.2mM solution of DPPH in methanol was prepared. World Scientific News 171 (2022) 1-20 -3- Gmelina arborea extracts to cure diarrhea.This study aims to examine the phytochemicals and in vitro antioxidant activity of Gmelina arborea leaf extracts.Moreover, to determine the phytochemicals that are responsible for therapeutic effects, HPTLC was used to profile Antioxidative activities of plant extracts were found
The extracts of A. cordifolia were screened for their phytochemicalss by standard procedures, and examined for antioxidant activity using various in vitro models: 2, 2'-azino-bis (3 . The scavenging activity of the pure antioxidant standard, ascorbic acid was 46% at lower
Aqueous extracts of turmeric showed antioxidant and antimicrobial activity due to the presence of curcumin (5%), a polyphenolic compound. Leaves | Context: Oxidative stress and . and Lobaria pulmonaria (L.) Hoffm. The hexane/acetone extracts were by far the best antimicrobial agent . This study aimed to investigate in vitro antidiabetic and antioxidant activity of three plants from Ficus species and effect of extracting solvents, total flavonoids and phenolics content on its in vitro activity. Herein, the CMF ethanol extract was also evaluated for antioxidant activity. Sansevieria trifasciata A. leaves and B. rhizomes In this study, the potential in vitro antioxidant activity of S.trifasciata aqueous and ethanolic leaf extracts was determined using DPPH assay for free radical scavenging activity and Antioxidant and reactive oxygen/nitrogen species' (ROS/RNS) inhibitory activity by the extracts was investigated using non-cell-based and cell-based assays.