In another study, Blando et al. extract or standard).. 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS) scavenging activity. Keywords: Extract, Free radical, Antioxidant activity, Medicinal plant. The stock solution of extracts were prepared in methanol to achieve the The high AA in the non-hydrolyzed extracts of these plants may be due to the presence of numerous volatile compounds . The antioxidant activity of the four plant extracts was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) to determine 50% inhibition of DPPH radical scavenging activity and reducing power by phosphomolybdate assay. These plant extracts also showed significant antioxidant activity with the IC50 ranges from 13.2 to 26.5 g/mL along with the significant antibacterial activity towards Staphylococcus aureus and . In addition, we compared the results obtained from the whole plant extracts with the results of analysis of extracts from different plant parts such as leaves, flowers, and stems and assessed the significance of particular analysis for effective use in pharmacy. . The plant extracts, in their majority do not present a unique phenolic component, but, on the contrary, correspond to the mixture of several of these. Although the methanol extract of U. longissima had a lower phenolic content (38.6 mg/g lyophylisate), it exhibited potent antioxidant activity. The two extracts also showed good antioxidant activity and total phenolic content as . The antioxidant activity of the plant extracts was examined on the basis of the scavenging effect on the stable DPPH free radical activity (Braca et al., 2002). Figure 3: Correlation between antioxidant activity and polyphenols (TPC and TFC) (A) Methanolic (B) Ethanolic (C) Aqueous extract of A. paniculata. Our study reports that the whole plant extract of A. paniculata plant is a rich source of natural antioxidants. Antioxidant activity on linoleic acid peroxidation The antioxidant activity of plant extracts against peroxidation of linoleic acid was Extract yield (%) Traditional indications Part used English name Family name Scientific name 13.0 Stomach pain, Disinfectant Flower yarrow Compositae Achillea tenuifolia Lam. There is a large variety of in vitro methods to quantify antioxidant activity, and it is important to select the proper method to determine which species have the . Experimental evidence suggests that most herbs and spices possess a wide range of biological and pharmacological activities that may protect tissues against O 2-induced damage.The objectives of the present study were: first, to determine the effects of plant extracts on the viability, membrane integrity, antioxidant status and DNA integrity of Caco-2 cells and second, to investigate the . Grape seed, pomegranate seed, green tea, and black tea extracts were used to inhibit the offodor from citral degradation. Among the partitions, the more polar ones (ethyl acetate and n -butanol) are those that generally have higher antioxidant activity (AA). The yield of the methanol extract of the various plant The plants were collected from Tirumala Hills, Tirupati, Chittoor district of Andhra Pradesh in the month of July - October 2008 and . In addition, the chemical composition of the four sample extracts was investigated using GC-MS. The method of Re et al. The free radical scavenging activity of the plant leaf extract was determined according to the method described by Brand William et al. 3.3. Abstract. Many epidemiological studies suggest that an increased consumption ofseveral medicinal plants containing antioxidants can protect against DNA damage andcarcinogenesis, and often exhibit a wide range of pharmacological activities such asantiflammatory, anti-bacterial, and anti-fungal properties []. Antioxidant properties and total phenolic . Extracts of plants contain many chemical compounds (7). and in vitro antioxidant activities of the whole plant extract of Chrozophora prostrata (Suryavarta, Neel kanthi, Shad, Khudi okra) was evaluated. Results show the importance of selecting the proper antioxidant activity quantification method for establishing a ranking of species based on this parameter, and show the best discrimination of differences and/or similarities between species is considered. (Original Article, Report) by "Advances in Environmental Biology"; Environmental issues Antioxidants Properties Antioxidants (Nutrients) Bioflavonoids Flavones Flavonoids Garlic Chemical properties Health aspects Liquid chromatography Materia medica, Vegetable Methanol Plant extracts There are many methods of evaluating the antioxidant activity of plant extracts, and in this study we have chosen to evaluate the antioxidant activity of Plectranthus aromaticus extracts using two different methods: the method of trapping the free radical DPPH (2.2-diphenyl-1-picrylhydrazyl) and the method of iron reduction FRAP (Ferric . Food Chem. Total phenolic content was also determined by the FolinCiocalteu method. Diabetes is a disease affecting health and economic system. Among edible plant materials, remarkable high antioxidant activity and high total phenolic content (GAE > 20 mg/g) were found in berries, especially aronia and crowberry. Table 1. Phytochemical profiling and antioxidant activity. . Compounds of the depsid structure of lecanoric acid, obtusic acid, and atranorin as well as usnic acid with a dibenzofuran structure were identified in the . where Abs control is the absorbance of DPPH + methanol; Abs sample is the absorbance of DPPH radical + sample (i.e. Plants have a large number of bioactive compounds with high antioxidant activity. Based on our results, we can say that as a general rule the ethanol extracts of plants belonging to the Verbenaceae family showed lower EC 50 values than the other plant extracts. S. elaeagnifolium has shown molluscicidal and nematicidal and cancer-inhibitory effects, anti-inflammatory, analgesic activity, and antibacterial activity. So far, in India, experimental studies have been conducted by a group of scientists, Anitha et al . [] was adopted for the determination of ABTS activity of the plant extract.The working solution was prepared by mixing two stock solutions of 7 mM . In vitro antioxidant activities of the whole plant extract of Chrozophora . Antioxidant properties of crude extract, partition extract, and fermented medium from Dendrobium sabin (DS) flower were investigated. Our result revealed significant antioxidant activities in both extracts and positive control and the values were noted higher with mounting concentration. Our results revealed that the total flavonoid content of methanol and chloroform extracts is 2.335 0.0097 and 1.7312 0.0082 mgQE/100 g respectively. The studies conducted on the antioxidant activities of some plants as natural antioxidants generally focused on the herbs and aromatic plants [4-7]. The aqueous extracts of Semen persicae, Leonurus cardiaca, Hedyotis diffusa . The parsley extract was the most abundant source of polyphenol compounds and showed the highest value of antioxidant activity in the group of the studied extracts. Extract of various solvents (chloroform, methanol, n-butanol and water) were prepared using cold maceration method. Introduction The many number of medicinal plants are used in the cellular and metabolic disease . DPPH scavenging assay is known to natural product researchers a most valued procedure to assess the antioxidant activity of the plant extracts. Antioxidant activity and increasing index were calculated using the following equations: This pattern was consistent in all 3 solvent systems (BEA, EMW and . The antioxidant activity of the decocted extracts of these five plants was studied by several methods including the DPPH tests, the ABTS test and the lipid peroxidation test. Antioxidant activity test-Carotene-linoleic acid emulsion system was used to determine the antioxidant activity of the extracts obtained from the three samples (Taga, Miller, & Pratt, 1984). Total phenolic content of the studied plant extracts was correlated with the DPPH radical scavenging activity. Solanum elaeagnifolium is among the invasive plants of Morocco; studies on its chemical composition and biological activities are few in number in Morocco. The antioxidant activity of the aerial part extract of M. quadrifolia was determined using the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay by the method of Blois (1958). There is a large variety of in vitro methods to quantify antioxidant activity, and it is important to select the proper method to . Thirty (30) plants aqueous extracts were investigated for their antioxidant properties via several methods such as DPPH, ABTS radical scavenging capacity assay, oxygen radical absorbance capacity (ORAC) assay, superoxide dismutase (SOD) assay . The . Sowndharrajan et al. The reaction mixtures were analyzed by highperformance liquid chromatography (HPLC) at days 0, 6, 10, 13, and 16 to monitor degradation . [ 12 ] also reported that the acetone extract from the stem bark of Acacia species effectively inhibited H 2 O 2 -mediated oxidative stress and may be a useful source as a therapeutic agent to prevent . The results showed that Ziziphus leaves presented the highest total antioxidant capacity (30~31 mg GAE/g DW) compared with other plant organs. The interest of our work lies in the study of the antioxidant activity of five plants including Psidium guajava L., Euphorbia hirta L., Combretum micranthum G. Don . 2010). Among polyphenols' interesting biological properties, their antioxidant activity is considered the most important. The acetone extracts of 27 cultivated plant species from Taiwan were tested for antioxidant activities towards xanthine oxidase, tyrosinase and lipoxygenase using spectrophotometric assays. In comparison to the previous results for the plant extracts WH, WT and RT, the WTEG retained activity in all three assays, inhibiting collagenase and elastase by over 10% when diluted to 1%, along with a TEAC score of > 5 moles (Table 1).In contrast, WHD at 10% exhibited a high anti-elastase activity of ca. Koelreuteria henryi, Prunus campanulata, and Rhodiola rosea showed the highest xanthine oxidase inhibitory activities. The reducing properties of the extracts are generally asso- Plants Res. The diabetic drugs are often reported to have side effects. pressure, the respective methanol extracts were obtained. References. These plants . Z. The antioxidant activities of methanol extracts of oregano, dittany, thyme, marjoram, spearmint, lavender and basil were tested in lard stored at 75C. Thus, we addressed this issue by evaluating the ability of teas from four different plants with therapeutic potential on gynecological diseases. DPPH assay also indicates the presence of phenolic and flavonoid compounds in plant extracts (Aryal et al., 2019) and is a popular mechanism to study the antioxidant property of plant extract. Little information is available concerning antioxidant effects of plant teas (water boiled) which are used more commonly in traditional Chinese medicine than other extracts. The in vitro antioxidant activity assays were carried out to assess the capacity of plant extracts to scavenge free radicals including 2,2azinobis(3ethylbenzothiazoline6sulfonic . The antioxidant activity of the and fresh rhubarb (Rheum rhabarbarum L.) stalks was investigated using four antioxidant assays. The research showed that each tested herb possesses its own fingerprint of phenolic compounds and antioxidant properties. . DPPH radical scavenging activity has been widely used to evaluate the antioxidant activity of plant extracts and foods . The aim of this study was to identify some of the secondary metabolites present in acetonic, methanolic, and hexanic extracts of lichen Xanthoparmelia stenophylla and to examine their antioxidant, antimicrobial, and cytotoxic activity. The compounds varied from polar to non-polar and in some extracts compounds of intermediate polarity were observed (Figure 1). Phytochemical components, including the phenolic and flavonoids, are important compounds that determine the plants antioxidant capacity, mainly due to their redox properties. Ethanolic solution of DPPH (0.05 mM) (300 l) was added to 40 l of extract solution with different concen- trations (0.02 - 2 mg/ml).